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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/11/2012 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
PIERRE MARRACCINI, Cenargen, CIRAD; FELIPE VINECKY, Cenargen; GABRIEL S. C. ALVES, Cenargen; HUMBERTO J. O. RAMOS, IAPAR; SONIA ELBELT, Cenargen, CIRAD; NATALIA G. VIEIRA, Cenargen; FERNANDA A. CARNEIRO, Cenargen; PATRICIA S. SUJII, Cenargen; JEAN C. ALEKCEVETCH, Cenargen; VÂNIA A. SILVA, UFV; FÁBIO M. DAMATTA, UFV; MARIA AMELIA GAVA FERRÃO, SAPC; THIERRY LEROY, CIRAD; DAVID POT, CIRAD; LUIZ G. E. VIEIRA, SAPC; FELIPE RODRIGUES DA SILVA, CNPTIA; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford, v. 63, n. 11, p. 4191-4212, 2012. |
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DOI: |
10.1093/jxb/ers103 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Differential expression; Drought acclimation; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea Canephora; Genética. |
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Thesaurus Nal: |
Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03043naa a2200445 a 4500
001 1940133
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008 2012 bl uuuu u00u1 u #d
024 7 $a10.1093/jxb/ers103$2DOI
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aGenetics
650 $aCafé
650 $aCoffea Canephora
650 $aGenética
653 $aCandidate gene
653 $aDifferential expression
653 $aDrought acclimation
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca |
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| Registros recuperados : 18 | |
| 2. |  | DOMICIANO, G. P.; SANTOS, R. F. dos; ALEKCEVETCH, J. C.; CONCEIÇÃO, L. D. H. C. S. da; LAVIOLA, B. G.; ALVES, A. A. Avaliação de características fisiológicas em progênies de macaúba com vistas ao melhoramento genético da espécie. In: CONGRESSO BRASILEIRO DE MACAÚBA, 1., 2013, Patos de Minas, MG. Consolidação da cadeia produtiva: anais. Brasília, DF: MAPA, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 3. | | ALEKCEVETCH, J. C.; PASSIANOTTO, A. L.; DIAS, W. P.; SANTOS, A. B. dos; BELZILE, F.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V. Fine mapping of soybean genes involved in resistance to Meloidogyne javanica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz do Iguaçu. Melhoramento de plantas: projetando o futuro: anais. Maringá: SBMP, 2017. v. 1. E-book. p. 67.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 4. | | CARANHATO, A. L. H.; GIORDANI, W.; MARINHO, J. P.; REIS, R. R.; ALEKCEVETCH, J. C.; MARIN, S. R.; MARCOLINO-GOMES, J.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Identificação e avaliação da expressão de genes pertencentes a família CCCH zinc finger em duas cultivares de soja sob deficit hídrico. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 11., 2016, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2016. p. 104-110. (Embrapa Soja. Documentos, 373).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|  |
| 5. | | VIDAL, R. O.; ALEKCEVETCH, J. C.; LEROY, T.; DE BELLIS, F.; POT, D.; RODRIGUES, G. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. High-through put sequencing of CDNA shows that CV. Rubi and IAPAR59 of Coffea Arabica have different molecular response to water privation. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | ALEKCEVETCH, J. C.; DOMICIANO, G. P; SANTOS, R. F. dos; VIEIRA, L. M.; CONCEIÇÃO, L. D. H. C. S. da; LAVIOLA, B. G.; ALVES, A. A. Correlação entre características morfológicas e fisiológicas em progênies de macaúba. In: CONGRESSO BRASILEIRO DE MACAÚBA, 1., 2013, Patos de Minas, MG. Consolidação da cadeia produtiva: anais. Brasília, DF: MAPA, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | CARNEIRO, F. A.; MENDONÇA, L. M. C.; ALEKCEVETCH, J. C.; VIEIRA, N. G.; COSTA, T. S.; FERRAO, M. A. G.; SILVA, V. A.; MARRACCINI, P.; ANDRADE, A. C. Rootomics: the first high-throughtput sequencing of cDNA extracted from roots of different clones of Coffea Canephora var. Conilon submitted to drought. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 223.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | ALEKCEVETCH, J. C.; CARNEIRO, F. de A.; RÊGO, E. C. da S.; GUERRA, A. F.; BARTHOLO, G. F.; FERRAO, M. A. G.; FONSECA, A. F. A. da; MARRACCINI, P.; ANDRADE, A. C. Estudo da diversidade genética de uma população de Coffea canephora VAR. Conilon por meio de marcadores moleculares do tipo SSR. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 9. | | VIEIRA, N. G.; ALVES, G. S. C.; FREIRE, L. P.; CARNEIRO, F. A.; MENDONÇA, L. M. C.; COSTA, T. S.; ALEKCEVETCH, J. C.; TAQUITA, J. A.; BLOCH JUNIOR, C.; MARRACCINI, P.; ANDRADE, A. C. No-hitomics: understanding the coffee unknowns. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 223.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | ALEKCEVETCH, J. C.; PASSIANOTTO, A. L. de L.; FERREIRA, E. G. C.; SANTOS, A. B. dos; SILVA, D. C. G. da; DIAS, W. P.; BELZILE, F.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genome-wide association study for resistance to the Meloidogyne javanica causing root-knot nematode in soybean. Theoretical and Applied Genetics, v. 134, p. 777-792, 2021. Artigo de acesso aberto.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; FREIRE, L. P.; VINECKY, F.; ELBELT, S.; SILVA, V. A.; MATTA, F. M. da; FERRAO, M. A. G.; MARRACCINI, P.; ANDRADE, A. C. Different molecular mechanisms account for drought tolerance in Coffea canephora var. Conilon. Tropical Plant Biology, v. 6, p. 181-190, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | CARNEIRO, F. A.; MENDONÇA, L. M. C.; ALEKCEVETCH, J. C.; VIEIRA, N. G.; COSTA, T. S.; FERRAO, M. A. G.; SILVA, V. A.; MARRACCINI, P.; ANDRADE, A. C.; FERRAO, M. A. G. Rootomics: the first high-throughtput sequencing of cDNA extracted from roots of different clones of Coffea Canephora var. Conilon submitted to drought. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 223. P. 223| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 13. | | REIS, R. R.; DIAS, B. B. A.; MARTINS, P. K.; MARTINS, M. T. B.; ALEKCEVETCH, J. C.; JÚNIOR, A. C.; ANDRADE, A. C.; RIBEIRO, A. P.; QIND, F.; MIZOI, J.; SHINOZAKI, K. I.; NAKASHIMA, K.; CARVALHO, J. de F. C.; SOUSA, C. A. F. de; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Induced over-expression of AtDREB2A CA improves drought tolerancein sugarcane. Plant Science, v. 221-222, p. 59-68, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | REIS, R. R.; CUNHA, B. A. D. B. da; MARTINS, P. K.; MARTINS, M. T. B.; ALEKCEVETCH, J. C.; CHALFUN-JÚNIOR, A.; ANDRADE, A. C.; RIBEIRO, A. P.; QIN, F.; MIZOI, J.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; CARVALHO, J. de F. C.; SOUSA, C. A. F. de; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Induced over-expression of AtDREB2A CA improves drought tolerance in sugarcane. Plant Science, Limerick, v. 221-222, p. 59-68, May, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | REIS, R. R.; DIAS, B. B. A.; MARTINS, P. K.; MARTINS, M. T. B.; ALEKCEVETCH, J. C.; JÚNIOR, A. C.; ANDRADE, A. C.; RIBEIRO, A. P.; QIND, F.; MIZOI, J.; SHINOZAKI, K. I.; NAKASHIMA, K.; CARVALHO, J. de F. C.; SOUSA, C. A. F. de; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Induced over-expression of AtDREB2A CA improves drought tolerance in sugarcane. Plant Science, v. 221-222, p. 59-68, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | MOFATTO, L. S.; CARNEIRO, F. de A.; VIEIRA, N. G.; DUARTE, K. E.; VIDAL, R. O.; ALEKCEVETCH, J. C.; COTTA, M. G.; VERDEIL, J-L.; LAPEYRE-MONTES, F.; LARTAUD, M.; LEROY, T.; DE BELLIS, F.; POT, D; RODRIGUES, G. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars. BMC Plant Biology, v. 16, n. 94, p. 1-18, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Café. |
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| 17. | | MARRACCINI, P.; VIEIRA, N. G.; DUARTE, K. E.; AQUINO, S. O.; CARNEIRO, F. A.; COSTA, T. S.; SUJII, P. S.; VINECKY, F.; ALEKCEVETCH, J. C.; ALVES, G. S. C.; LEROY, T.; BELLIS, F. de; FERRÃO, M. A. G.; DAMATTA, F. M.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C. Molecular responses of coffee plants to drought stress. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilheus. The challenge for the 21st century: book of abstracts. Ilhéus: International Advanced Biology Consortium, 2013. p. 29. Resumo S04T04.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 18. | | MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. Journal of Experimental Botany, Oxford, v. 63, n. 11, p. 4191-4212, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 18 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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